Effects of parabolic flight on redox status in SH-SY5Y cells / 生理学报

Space flight is known to produce a number of neurological disturbances. The etiology is unknown, but it may involve increased oxidative stress. A line of experimental evidence indicates that space flight may disrupt antioxidant defense system and result in increased oxidative stress. In vitro studies found that abundant of NO was produced in rat pheochromocytoma (PC12) cells, SHSY5Y neuroblastoma cells, and protein nitration was increased in PC12 cells within a simulated microgravity rotating wall bioreactor high aspect ratio vessel system or clinostat system. In the present study, we observed the change of redox status in SH-SY5Y cells after parabolic flight, and studied the effects of key redox molecule, thioredoxin (TRX), during the altered gravity. SH-SY5Y cells were divided into four groups: control cells, control cells transfected with TRX, flight cells and flight cells transfected with TRX. The expression levels of 3-nitrotyrosine (3-NT), inducible nitric oxide synthase (iNOS), TRX and thioredoxin reductase (TRXR) were observed by immunocytochemical method. It was shown that after parabolic flight, the staining of 3-NT and TRX were enhanced, while the expression level of TRXR was down-regulated compared with control. As for flight cells transfected with TRX, the staining of 3-NT and iNOS were weakened compared with flight cells. These results obtained suggest that altered gravity may increase protein nitration, down-regulate TRXR and elicit oxidative stress in SH-SY5Y cells, while TRX transfection could partly protect cells against oxidative stress induced by parabolic flight.

Frequency of the "Gravity Transition Effect" during Aerial Combat Mission of F-16 Aircraft, ROKAF / 항공우주의학회지

BACKGROUND: Current studies have focused mainly on the push-pull effect (PPE), the reduction of +Gz tolerance when hypergravity (>+1 Gz) preceded by hypogravity (<+1 Gz). However, the reduced G tolerance could be induced by any G-transition. The frequency and extent of maneuvers to cause G-transition effect (GTEM) have not been studied previously in fighter aircraft. METHODS: 26 HUD (head-up display) videotapes from F-16 aerial combat training missions were reviewed for the presence of GTEM. The frequency and magnitude of the +Gz profiles were analyzed. RESULTS: GTEMs were found in 34 (29.6%) of 115 engagements reviewed. PPEMs (maneuvers found to cause push-pull effect) and rPPEMs (maneuvers found to cause reversed PPE) were found in 7.8%, 11.3% respectively. Combined maneuver that rPPEM followed by PPEM consecutively was found in 10.4% of engagements. There was no difference the frequency of GTEMs between BFM and ACM engagements (28.8% vs. 30.9%). CONCLUSION: GTEMs including PPEMs and rPPEMs are present in aerial combat training missions of F-16 aircraft and represent a significant source for accidents. These findings support the necessity of continued research into the physiologic response to GTE.